Label-free classification of cultured cells through diffraction imaging
نویسندگان
چکیده
Automated classification of biological cells according to their 3D morphology is highly desired in a flow cytometer setting. We have investigated this possibility experimentally and numerically using a diffraction imaging approach. A fast image analysis software based on the gray level co-occurrence matrix (GLCM) algorithm has been developed to extract feature parameters from measured diffraction images. The results of GLCM analysis and subsequent classification demonstrate the potential for rapid classification among six types of cultured cells. Combined with numerical results we show that the method of diffraction imaging flow cytometry has the capacity as a platform for high-throughput and label-free classification of biological cells.
منابع مشابه
Pattern recognition and classification of two cancer cell lines by diffraction imaging at multiple pixel distances
Rapid and label-free imaging methods for accurate cell classification are highly desired for biology and clinical research. To improve consistency of classification performance, we have developed an approach of pattern analysis by gray level co-occurrence matrix (GLCM) algorithm to extract textural features at multiple pixel distances from cross-polarized diffraction image (p-DI) pairs, which w...
متن کاملDynamic nano-imaging of label-free living cells using electron beam excitation-assisted optical microscope
Optical microscopes are effective tools for cellular function analysis because biological cells can be observed non-destructively and non-invasively in the living state in either water or atmosphere condition. Label-free optical imaging technique such as phase-contrast microscopy has been analysed many cellular functions, and it is essential technology for bioscience field. However, the diffrac...
متن کاملDepth-resolved mid-infrared photothermal imaging of living cells and organisms with submicrometer spatial resolution
Chemical contrast has long been sought for label-free visualization of biomolecules and materials in complex living systems. Although infrared spectroscopic imaging has come a long way in this direction, it is thus far only applicable to dried tissues because of the strong infrared absorption by water. It also suffers from low spatial resolution due to long wavelengths and lacks optical section...
متن کاملAssessment of metrezoate-labeled gallium transmission in cultured human Burkitt lymphatic cells
Background: In addition to determining the exact tumor location and its geometric features, an increase of the effective tumor atomic number will enhances the chance in treating the tumoral cells under suitable radiation. In the present study, we assessed metrezoate-labeled gallium transmission in Burkett lymphatic cells. Materials and Methods: Human Burkitt lymphatic cells were grown ...
متن کاملWhite-light diffraction tomography of unlabelled live cells
We present a technique called white-light diffraction tomography (WDT) for imaging microscopic transparent objects such as live unlabelled cells. The approach extends diffraction tomography to white-light illumination and imaging rather than scattering plane measurements. Our experiments were performed using a conventional phase contrast microscope upgraded with a module to measure quantitative...
متن کامل